NGS-Based RNA-Sequencing Market Size, Share & Trends Analysis Distribution by Type (Targeted RNA Sequencing, mRNA Sequencing, Total RNA Sequencing, Single-cell RNA Sequencing (scRNA-Seq), De Novo Transcriptome Assembly , and Non-coding RNA Sequencing (miRNA, lncRNA, circRNA)), Workflow Component, Technology, Application, End-user, and Segment Forecasts, 2025-2034

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Segmentation of NGS-Based RNA-Sequencing Market -

NGS-Based RNA-Sequencing Market by Type-

·         Targeted RNA Sequencing

·         mRNA Sequencing

·         Total RNA Sequencing

·         Single-cell RNA Sequencing (scRNA-Seq)

·         De Novo Transcriptome Assembly 

·         Non-coding RNA Sequencing (miRNA, lncRNA, circRNA)

NGS-Based RNA-Sequencing Market
NGS-Based RNA-Sequencing Market by Workflow Component -

·         Sequencing

·         Library Preparation & Sample Processing

·         Storage & Cloud Bioinformatics

·         Data Analysis & Interpretation

NGS-Based RNA-Sequencing Market by Technology-

·         Sequencing by Synthesis (SBS)

·         Third-generation Sequencing (PacBio, ONT) 

·         Ion Semiconductor Sequencing 

·         Nanopore Sequencing (Oxford Nanopore)

·         SOLiD & Pyrosequencing (legacy/declining)

NGS-Based RNA-Sequencing Market by Application-

·         Precision Medicine

·         Drug Discovery & Development

·         Cancer Genomics

·         Disease Mechanism Studies

·         Biomarker Discovery 

·         Single-Cell Transcriptomics in Immuno-Oncology

·         Plant and Microbial Transcriptomics 

NGS-Based RNA-Sequencing Market by End-user-

·         Pharmaceutical & Biotechnology Companies

·         Academic & Research Institutions

·         Contract Research Organizations (CROs) 

·         Government and Non-profit Organizations

·         Clinical & Diagnostic Laboratories

NGS-Based RNA-Sequencing Market by Region-

North America-

·         The US

·         Canada

Europe-

·         Germany

·         The UK

·         France

·         Italy

·         Spain

·         Rest of Europe

Asia-Pacific-

·         China

·         Japan

·         India

·         South Korea

·         Southeast Asia

·         Rest of Asia Pacific

Latin America-

·         Brazil

·         Argentina

·         Mexico

·         Rest of Latin America

 Middle East & Africa-

·         GCC Countries

·         South Africa

·         Rest of the Middle East and Africa

Chapter 1.    Methodology and Scope
1.1.    Research Methodology
1.2.    Research Scope & Assumptions

Chapter 2.    Executive Summary

Chapter 3.    Global NGS-Based RNA-Sequencing Market Snapshot

Chapter 4.    Global NGS-Based RNA-Sequencing Market Variables, Trends & Scope
4.1.    Market Segmentation & Scope
4.2.    Drivers
4.3.    Challenges
4.4.    Trends 
4.5.    Investment and Funding Analysis 
4.6.    Porter's Five Forces Analysis
4.7.    Incremental Opportunity Analysis (US$ MN), 2025-2034 
4.8.    Competitive Landscape & Market Share Analysis, By Key Player (2024)
4.9.    Use/impact of AI on NGS-Based RNA-Sequencing Market Industry Trends 
4.10.    Global NGS-Based RNA-Sequencing Market Penetration & Growth Prospect Mapping (US$ Mn), 2024-2034

Chapter 5.    NGS-Based RNA-Sequencing Market Segmentation 1: By Type, Estimates & Trend Analysis
5.1.    Market Share by Type, 2024 & 2034
5.2.    Market Size Revenue (US$ Million) & Forecasts and Trend Analyses, 2021 to 2034 for the following Type:

5.2.1.    mRNA Sequencing

5.2.1.1.    Analysis of coding transcripts, widely used in disease research

5.2.2.    Total RNA Sequencing
5.2.3.    Targeted RNA Sequencing
5.2.4.    Single-cell RNA Sequencing (scRNA-Seq)

5.2.4.1.    Enables high-resolution transcriptomic profiling of individual cells

5.2.5.    Non-coding RNA Sequencing (miRNA, lncRNA, circRNA)
5.2.6.    De Novo Transcriptome Assembly 

Chapter 6.    NGS-Based RNA-Sequencing Market Segmentation 2: By End-User, Estimates & Trend Analysis
6.1.    Market Share by End-User, 2024 & 2034
6.2.    Market Size Revenue (US$ Million) & Forecasts and Trend Analyses, 2021 to 2034 for the following End-User:

6.2.1.    Academic & Research Institutions
6.2.2.    Pharmaceutical & Biotechnology Companies
6.2.3.    Contract Research Organizations (CROs) 
6.2.4.    Clinical & Diagnostic Laboratories 
6.2.5.    Government and Non-profit Organizations

Chapter 7.    NGS-Based RNA-Sequencing Market Segmentation 3: By Application, Estimates & Trend Analysis
7.1.    Market Share by Application, 2024 & 2034
7.2.    Market Size Revenue (US$ Million) & Forecasts and Trend Analyses, 2021 to 2034 for the following Application:

7.2.1.    Drug Discovery & Development
7.2.2.    Disease Mechanism Studies
7.2.3.    Biomarker Discovery 
7.2.4.    Precision Medicine
7.2.5.    Cancer Genomics
7.2.6.    Single-Cell Transcriptomics in Immuno-Oncology
7.2.7.    Plant and Microbial Transcriptomics 

Chapter 8.    NGS-Based RNA-Sequencing Market Segmentation 4: By Workflow Component, Estimates & Trend Analysis
8.1.    Market Share by Workflow Component, 2024 & 2034
8.2.    Market Size Revenue (US$ Million) & Forecasts and Trend Analyses, 2021 to 2034 for the following Workflow Component:

8.2.1.    Library Preparation & Sample Processing
8.2.2.    Sequencing
8.2.3.    Data Analysis & Interpretation
8.2.4.    Storage & Cloud Bioinformatics

Chapter 9.    NGS-Based RNA-Sequencing Market Segmentation 5: By Technology, Estimates & Trend Analysis
9.1.    Market Share by Technology, 2024 & 2034
9.2.    Market Size Revenue (US$ Million) & Forecasts and Trend Analyses, 2021 to 2034 for the following Technology:

9.2.1.    Sequencing by Synthesis (SBS)

9.2.1.1.    Illumina platforms, high throughput 

9.2.2.    Nanopore Sequencing (Oxford Nanopore)
9.2.3.    Ion Semiconductor Sequencing 
9.2.4.    Third-generation Sequencing (PacBio, ONT) 
9.2.5.    SOLiD & Pyrosequencing (legacy/declining)

Chapter 10.    NGS-Based RNA-Sequencing Market Segmentation 6: Regional Estimates & Trend Analysis
10.1.    Global NGS-Based RNA-Sequencing Market, Regional Snapshot 2024 & 2034
10.2.    North America

10.2.1.    North America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Country, 2021-2034

10.2.1.1.    US
10.2.1.2.    Canada

10.2.2.    North America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Type, 2021-2034
10.2.3.    North America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by End-User, 2021-2034
10.2.4.    North America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Application, 2021-2034
10.2.5.    North America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Workflow Component, 2021-2034
10.2.6.    North America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Technology, 2021-2034

10.3.    Europe

10.3.1.    Europe NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Country, 2021-2034

10.3.1.1.    Germany
10.3.1.2.    U.K.
10.3.1.3.    France
10.3.1.4.    Italy
10.3.1.5.    Spain
10.3.1.6.    Rest of Europe

10.3.2.    Europe NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Type, 2021-2034
10.3.3.    Europe NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by End-User, 2021-2034
10.3.4.    Europe NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Application, 2021-2034
10.3.5.    Europe NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Workflow Component, 2021-2034
10.3.6.    Europe NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Technology, 2021-2034

10.4.    Asia Pacific

10.4.1.    Asia Pacific NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Country, 2021-2034

10.4.1.1.    India 
10.4.1.2.    China
10.4.1.3.    Japan
10.4.1.4.    Australia
10.4.1.5.    South Korea
10.4.1.6.    Hong Kong
10.4.1.7.    Southeast Asia
10.4.1.8.    Rest of Asia Pacific

10.4.2.    Asia Pacific NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Type, 2021-2034
10.4.3.    Asia Pacific NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by End-User, 2021-2034
10.4.4.    Asia Pacific NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Application, 2021-2034
10.4.5.    Asia Pacific NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Workflow Component, 2021-2034
10.4.6.    Asia Pacific NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Technology, 2021-2034

10.5.    Latin America

10.5.1.    Latin America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Country, 2021-2034

10.5.1.1.    Brazil
10.5.1.2.    Mexico
10.5.1.3.    Rest of Latin America

10.5.2.    Latin America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Type, 2021-2034
10.5.3.    Latin America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by End-User, 2021-2034
10.5.4.    Latin America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Application, 2021-2034
10.5.5.    Latin America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Workflow Component, 2021-2034
10.5.6.    Latin America NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Technology, 2021-2034

10.6.    Middle East & Africa 

10.6.1.    Middle East & Africa Wind Turbine Rotor Blade Market Revenue (US$ Million) Estimates and Forecasts by country, 2021-2034

10.6.1.1.    GCC Countries
10.6.1.2.    Israel
10.6.1.3.    South Africa
10.6.1.4.    Rest of Middle East and Africa

10.6.2.    Middle East & Africa NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Type, 2021-2034
10.6.3.    Middle East & Africa NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by End-User, 2021-2034
10.6.4.    Middle East & Africa NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Application, 2021-2034
10.6.5.    Middle East & Africa NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Workflow Component, 2021-2034
10.6.6.    Middle East & Africa NGS-Based RNA-Sequencing Market Revenue (US$ Million) Estimates and Forecasts by Technology, 2021-2034

Chapter 11.    Competitive Landscape
11.1.    Major Mergers and Acquisitions/Strategic Alliances
11.2.    Company Profiles
 

11.2.1.     Illumina, Inc.

11.2.1.1.    Business Overview
11.2.1.2.    Key Type/Service Overview
11.2.1.3.    Financial Performance
11.2.1.4.    Geographical Presence
11.2.1.5.    Recent Developments with Business Strategy

11.2.2.    Thermo Fisher Scientific 
11.2.3.    Oxford Nanopore Technologies
11.2.4.    Pacific Biosciences (PacBio)
11.2.5.    Agilent Technologies
11.2.6.    QIAGEN
11.2.7.    Takara Bio Inc.
11.2.8.    10x Genomics
11.2.9.    BGI Genomics
11.2.10.    PerkinElmer (Revvity)
11.2.11.    CD Genomics
11.2.12.    Novogene Corporation
11.2.13.    Eurofins Genomics
11.2.14.    Fulgent Genetics
11.2.15.    Macrogen Inc.
11.2.16.    Bio-Rad Laboratories
11.2.17.    Genewiz (Azenta Life Sciences)
11.2.18.    Parse Biosciences
11.2.19.    Element Biosciences  

Research Design and Approach

This study employed a multi-step, mixed-method research approach that integrates:

  • Secondary research
  • Primary research
  • Data triangulation
  • Hybrid top-down and bottom-up modelling
  • Forecasting and scenario analysis

This approach ensures a balanced and validated understanding of both macro- and micro-level market factors influencing the market.

Secondary Research

Secondary research for this study involved the collection, review, and analysis of publicly available and paid data sources to build the initial fact base, understand historical market behaviour, identify data gaps, and refine the hypotheses for primary research.

Sources Consulted

Secondary data for the market study was gathered from multiple credible sources, including:

  • Government databases, regulatory bodies, and public institutions
  • International organizations (WHO, OECD, IMF, World Bank, etc.)
  • Commercial and paid databases
  • Industry associations, trade publications, and technical journals
  • Company annual reports, investor presentations, press releases, and SEC filings
  • Academic research papers, patents, and scientific literature
  • Previous market research publications and syndicated reports

These sources were used to compile historical data, market volumes/prices, industry trends, technological developments, and competitive insights.

Secondary Research

Primary Research

Primary research was conducted to validate secondary data, understand real-time market dynamics, capture price points and adoption trends, and verify the assumptions used in the market modelling.

Stakeholders Interviewed

Primary interviews for this study involved:

  • Manufacturers and suppliers in the market value chain
  • Distributors, channel partners, and integrators
  • End-users / customers (e.g., hospitals, labs, enterprises, consumers, etc., depending on the market)
  • Industry experts, technology specialists, consultants, and regulatory professionals
  • Senior executives (CEOs, CTOs, VPs, Directors) and product managers

Interview Process

Interviews were conducted via:

  • Structured and semi-structured questionnaires
  • Telephonic and video interactions
  • Email correspondences
  • Expert consultation sessions

Primary insights were incorporated into demand modelling, pricing analysis, technology evaluation, and market share estimation.

Data Processing, Normalization, and Validation

All collected data were processed and normalized to ensure consistency and comparability across regions and time frames.

The data validation process included:

  • Standardization of units (currency conversions, volume units, inflation adjustments)
  • Cross-verification of data points across multiple secondary sources
  • Normalization of inconsistent datasets
  • Identification and resolution of data gaps
  • Outlier detection and removal through algorithmic and manual checks
  • Plausibility and coherence checks across segments and geographies

This ensured that the dataset used for modelling was clean, robust, and reliable.

Market Size Estimation and Data Triangulation

Bottom-Up Approach

The bottom-up approach involved aggregating segment-level data, such as:

  • Company revenues
  • Product-level sales
  • Installed base/usage volumes
  • Adoption and penetration rates
  • Pricing analysis

This method was primarily used when detailed micro-level market data were available.

Bottom Up Approach

Top-Down Approach

The top-down approach used macro-level indicators:

  • Parent market benchmarks
  • Global/regional industry trends
  • Economic indicators (GDP, demographics, spending patterns)
  • Penetration and usage ratios

This approach was used for segments where granular data were limited or inconsistent.

Hybrid Triangulation Approach

To ensure accuracy, a triangulated hybrid model was used. This included:

  • Reconciling top-down and bottom-up estimates
  • Cross-checking revenues, volumes, and pricing assumptions
  • Incorporating expert insights to validate segment splits and adoption rates

This multi-angle validation yielded the final market size.

Forecasting Framework and Scenario Modelling

Market forecasts were developed using a combination of time-series modelling, adoption curve analysis, and driver-based forecasting tools.

Forecasting Methods

  • Time-series modelling
  • S-curve and diffusion models (for emerging technologies)
  • Driver-based forecasting (GDP, disposable income, adoption rates, regulatory changes)
  • Price elasticity models
  • Market maturity and lifecycle-based projections

Scenario Analysis

Given inherent uncertainties, three scenarios were constructed:

  • Base-Case Scenario: Expected trajectory under current conditions
  • Optimistic Scenario: High adoption, favourable regulation, strong economic tailwinds
  • Conservative Scenario: Slow adoption, regulatory delays, economic constraints

Sensitivity testing was conducted on key variables, including pricing, demand elasticity, and regional adoption.

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Frequently Asked Questions

The NGS-Based RNA-Sequencing Market Size is valued at US$ 3.6 Bn in 2024 and is predicted to reach US$ 20.3 Bn by the year 2034 at an 19.2% CAGR over the forecast period.

The major players in the NGS-Based RNA-Sequencing market are Agilent Technologies, QIAGEN, Illumina, Inc., Thermo Fisher Scientific, Takara Bio Inc., 10x Genomics, Oxford Nanopore Technologies, Pacific Biosciences (PacBio), BGI Genomics, Novogene Corporation, Eurofins Genomics, Bio-Rad Laboratories, Genewiz (Azenta Life Sciences), Parse Biosciences, PerkinElmer (Revvity), CD Genomics, Fulgent Genetics, Macrogen Inc., and Element Biosciences.

The primary NGS-Based RNA-Sequencing market segments are Type, Workflow Component, Technology, Application, and End-user.

North America leads the market for NGS-Based RNA-Sequencing because of the area\'s early adoption of state-of-the-art genomic technologies, significant investment in research, and robust presence of leading sequencing technology companies.

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